Hydrogen sulfide content was quantified colorimetrically tissue detection kit product specification (Chinese version) The main purpose YIJI tissue hydrogen sulfide content colorimetric quantitative detection reagent is a change in the absorption peak caused by the reaction of basic picric acid with creatinine to produce a blue product, that is, the colorimetric method is used to determine the hydrogen sulfide content in the sample. An authoritative and classic technical approach. This technology has been proven through careful modification of the methylene blue method and successful experiments. It is suitable for the detection of hydrogen sulfide levels in various human and animal tissue lysis samples. The product is strictly sterile, ready to use, simple in operation and stable in performance. technical background Hydrogen sulfide (H2S) is a naturally occurring, corrosive and flammable, unstable, oxidizable, colorless gas that is a desulfuration metabolite of cysteine ​​and is the third physiology. A gas messenger molecule or a gasophore molecule exists in the brain, heart, lung, liver, small intestine, pancreas, and cavernosum tissues, in which brain tissue content is low and kidney tissue content is high. . Nervous system cystathionine -β - synthase (Cystathionine-β-synthase; CBS ) , and cardiovascular system cystathionine - γ- lyase (cystathionine γ -lyase; CGL) is involved in the generation of endogenous hydrogen sulfide main members. Once the body is deprived of oxygen, the hydrogen sulfide content increases. Hydrogen sulfide is intended to regulate the level of sulfur-containing amino acids in tissues, and has physiological functions and pathophysiological regulation of the cardiovascular system, such as induction of vascular smooth muscle relaxation, vasodilation, decreased blood pressure, and pro-inflammatory effects. At the same time, hydrogen sulfide has a toxic effect, which causes rapid convulsions of the olfactory nerve, inhibits cytochrome c oxidase, increases the opening of the mitochondrial membrane channel, and causes excessive relaxation of the heart, small intestine, reproductive system, urinary system and respiratory smooth muscle, resulting in organ damage, Hypotension, bradycardia and other symptoms. In the natural environment, hydrogen sulfide is the main intermediate of the sulfur cycle, including mineralization or decomposition of organic sulfides, assimilation of microorganisms, and redox. As a chemical asphyxiant gas, hydrogen sulfide is toxic to fish and other aquatic animals and is also a marker of bacterial contamination. Zinc acetate is used to capture hydrogen sulfide under alkaline conditions to form a stable zinc sulfide precipitate; under acidic conditions, dissolved zinc sulfide and catalyst p-aminodimethylaniline (N, N-dimethyl-p- Phenylenediamine reacts with ferric chloride to produce blue methylene blue, which is quantitatively analyzed for its hydrogen sulfide content by its peak absorption peak (wavelength at 670 nm). product content YIJI cleaning solution (Reagent A) 100 ml YIJI Stabilizer (Reagent B) 5 ml YIJI Decontamination Solution (Reagent C) 5 ml YIJI Reaction Solution (Reagent D) 2.5 ml YIJI Color Reagent (Reagent E) 2 ml YIJI standard solution (Reagent F) 1 ml Product manual 1 copy storage method Store YIJI reaction solution (Reagent D) and YIJI standard solution (Reagent F) in -20 °C refrigerator, and keep the rest in 4 °C refrigerator; YIJI reaction solution (Reagent D) to avoid light; reagent is corrosive, pay attention to operation Safe; effective guarantee for June User-supplied 1.5 ml centrifuge tube: container for sample handling 15 ml conical centrifuge tube: container for sample handling DOUNCE homogenizer: used to lyse tissue cells Micro Tabletop Centrifuge: for sample handling Cuvette: a container for colorimetry Spectrophotometer: for colorimetric analysis Experimental procedure Fourth, sample determination Fifth, calculate the sample concentration [(sample read sample X sample dilution factor) ÷ standard sample reading] X 200 micromoles / liter = micromoles hydrogen sulfide / liter Converted to the actual hydrogen sulfide content of the tissue Namol hydrogen sulfide / ml X 1 ml (tissue suspension) = nanomolar / 200 mg tissue Precautions Quality Standard Use commitment Shanghai Yiji adheres to the tenet of “Credit First, Customer Satisfaction, Quality Commitment†to provide quality products and services to our customers. After receiving the goods, the user should keep it in accordance with the provisions of the product manual and use it within the validity period. Our products have been rigorously qualified before sale to ensure the use of the instructions described in the manual. If it is found that the quality of this product is within 10 days after the arrival of the goods, please contact the company immediately in written form (experiment report) and return the product. The inspection is confirmed to be due to the quality of the product. . In the event of a user's incorrect operation, the company will not be liable for the losses caused. Color Doppler Ultrasound Scanner "Color Doppler Ultrasound Scanner" is a high-definition black-and-white B-ultrasound plus color Doppler. Its main advantages are: it can quickly and intuitively display the two-dimensional plane distribution of blood flow; it can display the running direction of blood flow; it is beneficial to distinguish between arteries and veins; it is beneficial to identify vascular disease and non-vascular disease; it is beneficial to understand the nature of blood flow ; It is convenient to understand the phase and speed of blood flow; it can reliably detect shunt and regurgitation; it can quantitatively analyze the origin, width, length and area of blood flow. These ultrasound manifestations and parameters play a very important auxiliary role in the diagnosis of clinical diseases. Color Doppler Ultrasound Scanner,Color Doppler Scanner,Color Doppler Ultrasonic,Laptop Ultrasonic Scanner Mianyang United Ultrasound Electronics Co., Ltd , https://www.uniultrasonic.com