Sample description Silk fibroin, a natural high-molecular fibrin extracted from silk, contains about 70% to 80% of silk, and contains 18 kinds of amino acids, of which glycine (Gly), alanine (Ala) and serine (Ser) are about It accounts for more than 80% of the total composition. Silk fibroin has good mechanical properties and physical and chemical properties, such as good flexibility and tensile strength, breathable moisture permeability, sustained release, etc., and different treatments can be obtained in different forms, such as fiber, solution, powder, film. And gels, etc. ---Instrument Configuration --- ---Reagents --- ---Instrument parameters --- ---Detection method--- ---Sample moisture test record --- sample name: Silk fibroin environment humidity: 61% Ambient temperature: 28 °C Drift correction: 0.406ul/s Titration: 2.588mg/ml Measurement order Injection volume Mg Titration volume mL Water content Mg Measuring time m:s The measurement results % 1 19.3 0.457 1.184 4:34 6.13356% 2 21.8 0.515 1.332 4:53 6.10939% 3 22.1 0.523 1.352 4:37 6.11899% Average %: 6.1206% RSD deviation %: 0.1988% Edible Mushroom Extract,Hericium Erinaceus Extract,Boletus Extract Fufeng Sinuote Biotechnology Co.,Ltd. , https://www.sntextract.com
In order to more accurately detect the moisture in silk fibroin, we use a mixed solution of ethanol calcium chloride solution and anhydrous methanol as a solvent for the titration system, mainly ethanol calcium chloride solution can dissolve silk fibroin, which is more conducive to the sample. The release of moisture increases the speed and accuracy of the entire reaction process.
1. S-300 Karl Fischer Moisture Analyzer host;
2. Fully enclosed safety titration cell assembly;
3. Double platinum electrode;
4. Titration tank mixing station;
5. 10ml syringe
6. 1ul syringe
7. Volumetric flask
8. Analyze one of the balances
Titrant: Karl Fischer reagent,
Solvent: absolute ethanol;
Auxiliary reagent: anhydrous ethanol, calcium chloride;
Control accuracy: 0.5ul
Stirring speed: 20%
Suction and discharge reagent: automatic auxiliary function: method save, the result is automatically calculated and stored, device verification, titration delay.
End current: 51μA
Titration delay: 180 seconds End point delay: 10 seconds drift deduction: not open drift value update: automatic delay setting: can set delay titration, end point delay function to respond to insoluble samples according to needs
1. Using the solvent absorption function of the instrument, inject about 50 ml of calcium chloride saturated absolute ethanol solvent into the titration cell.
2. Use the blank function of the instrument to titrate to the end point to remove the moisture in the titration cell and the instrument is ready.
3. Use a dry micro-needle to accurately extract 10u; L pure water, after drying the needle, select the instrument calibration function, inject pure water into the titration tank below the input liquid into the pure water weight 10mg, start calibration.
4. Repeat step 3 and repeat the measurement for 3~5 times. The instrument will automatically save the calibration result and calculate the average value as the titer of the reagent.
5. Use a sample of 20 mg for the month and set aside.
6. Select “Measure†and press the OK button. After weighing, inject the sample into the titration cell. In the injection interface, enter the sample injection weight, and then press the OK button, the instrument automatically starts measuring, and the result is automatically displayed after the end.