CompresstomeTM Application II Gene Functional Study: Histology Serial Slices

Keywords: Histology, Gene Function, Immunohistochemistry, Serial Sections, Vibratome

In the study of gene function , in order to study the function or gene regulatory network of a certain gene, it is necessary to simultaneously mutate multiple genes in one animal. This type of analysis of two or more genes requires the in situ hybridization of genes, the immunohistochemical technique of gene-expressed proteins to observe the effects of mutational operations at the developmental organ level or at the cellular level of tissue sections. With the advent of more advanced instruments, research techniques are constantly being updated. By binding the lipophilic tracer dye, in situ hybridization, immunohistochemistry and histology other technology changes in the data acquisition as well as structural changes in gene and protein expression levels in various tissues organs from the same animal to maximize such knock In addition , gene expression is more closely related to histological data.

Schematic diagram of experimental design: The following four technical operations were carried out in the same sample: 1. Dye tracing; 2. In situ hybridization showed mRNA expression; 3. Immunohistochemistry showed protein expression; 4. Histological section showed in situ hybridization And immuno-textile chemical staining results.

Note: The new CompresstomeTM vibrating slicer embeds the sample in the sample tube through agarose gel and applies a slight compression to make the tissue more secure, thus achieving better sectioning (more uniform slice thickness, sliced ​​surface) Smoother and smoother).

Related links: "Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology"
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