Autoclaving is to place the items to be sterilized in a closed pressurized sterilizing pot, and by heating, the water in the sterilizer is boiled to generate steam. Waiting for the steam to drastically drive the cold air in the pot out of the exhaust valve, then close the exhaust valve and continue heating. At this time, the steam can not overflow, and the pressure in the sterilizer is increased, so that the boiling point is increased. A temperature higher than 100 ° C is obtained. It causes the bacterial protein to coagulate and denature and achieve sterilization. MP Nucleic Acid Test Kit MP Nucleic Acid Test Kit Guangdong Hecin Scientific, Inc. , https://www.hecinscientific.com experimental method
Principle of experimental method When using a high-pressure steam sterilization pot, the elimination of cold air in the sterilization pot is completely important because the expansion pressure of the air is greater than the expansion pressure of the water vapor. Therefore, when the water vapor contains air, under the same pressure. The temperature of the air-containing steam is lower than the temperature of the saturated steam. Reagents, kits Instruments, consumables Experimental procedure
2. Replace the sterilization bucket and load the items to be sterilized. Be careful not to pack too much, so as not to hinder the circulation of steam and affect the sterilization effect. Do not contact the barrel end of the flask and the end of the tube to prevent the condensate from drenching the paper at the mouth and penetrating into the tampon.
3. Cap and insert the exhaust hose on the cover into the exhaust vent of the inner sterilization tank. Then tighten the two bolts at the same time in a symmetrical manner to make the bolts tight and not leaking.
4. Heat with an electric stove or gas and open the exhaust valve at the same time to boil the water to remove the cold air from the pot. After the cold air is completely drained, close the exhaust valve so that the temperature inside the pot gradually increases as the steam pressure increases. When the pressure in the pot rises to the desired pressure, the heat source is controlled to maintain the pressure for the required time. This experiment was sterilized with 1.05 kg/cm 2 at 121.3 ° C for 20 minutes.
5. After the time required for sterilization, turn off the power or turn off the gas, let the temperature inside the sterilization pot drop naturally. When the pressure of the pressure gauge drops to 0, open the exhaust valve, loosen the bolt, open the cover, and take out the sterilization. article. If the pressure does not drop to 0, open the exhaust valve, the pressure in the pot will suddenly drop, so that the medium in the container will be flushed out of the flask mouth or the test tube mouth due to the imbalance of internal and external pressure, causing the cotton plug to contaminate the medium. Pollution.
6. Incubate the removed sterilized medium in a 37 ° C incubator for 24 hours. Check for growth without bacteria.
Microbiology: High Pressure Steam Sterilization Experiment
Beef extract peptone medium
Petri dish portable high pressure steam fungus pot
1. First remove the inner layer sterilization barrel, and then add an appropriate amount of water to the outer layer pot to make the water surface and the triangle shelf level.