Zearalenone is an estrogen-like substance and the target organ is the reproductive organ of an animal. Toxin can cause estrogen hyperactivity in pigs, which can change the function and morphology of pig reproductive organs, leading to breast enlargement of gilts, premature breast maturation, redness of vagina and vulva, genital valgus, vaginal prolapse, etc. Inflammation. The boar testicular atrophy, decreased sexual desire, and lack of vitality of the sperm. At present, zearalenone antigen antibody is required for both zearalenone enzyme-linked immunosorbent assay and immunocolloidal gold assay. The best choice for Analog hearing aids with earbuds design The best choice for Analog hearing aids with earbuds design Shenzhen Sunshine Technology Co.,Ltd , https://www.shenzhenyatwin.com
(1) Zearalenone hapten synthesis (synthesis route is shown below)
10-20 mg of zearalenone is dissolved in acetone and reacted with sodium chloroacetate in an alkaline solution to form a zearalenone hapten containing an acetate group.
(2) Synthesis of zearalenone total antigen (artificial antigen, complete antigen, enzyme-labeled antigen) The zearalenone hapten is coupled with bovine serum albumin to obtain an immunizing antigen.
The zearalenone hapten was activated by EDC, and then 100 mg of protein was added to 0.01 mpbs, dissolved and conjugated to zearalenone hapten, stirred at room temperature for 1 h, dialyzed against 0.01 mol/L PBS for 3 days, and changed 3 times a day. The dialysate is used to obtain an immunizing antigen of zearalenone and bovine serum albumin. 