Product categories of Hot Melt Machine, we are specialized manufacturers from China,Four-column Hot Melt Machine,Saddle Type Hot Melt Machine suppliers/factory, wholesale high-quality machine of hot melt R & D and manufacturing, we have the perfect after-sales service and technical support. Look forward to your cooperation! Hot Melt Machine,Four-column Hot Melt Machine,Saddle Type Hot Melt Machine,Nut Hot Melt Hot Press Implanter Wuxi DIZO Ultrasonic Technology Company , https://www.dizosonic.com
The phenomenon of false positives in imported ELISA test kits is generally related to the wrong operation. This article will talk to you about how to avoid false positives.
How to avoid false positives in imported ELISA test kits
The phenomenon of false positives in imported ELISA test kits is generally related to incorrect operation.
1, loading
For indirect imported ELISA test kit specimens are generally diluted, if the sample is not allowed will cause errors, especially when the dilution factor is large, a small absolute error will lead to a large relative error, making the negative (or weak) Positive) specimens were positive (or negative). When adding the sample, add the added substance to the bottom of the hole of the ELISA plate, avoid adding it to the upper part of the hole wall, and pay attention to not spilling, and no bubble can be generated. At present, most blood stations have used the automatic enzyme-free sample loading system to process specimens, which can better avoid the above errors.
2, washing
Proper washing in an imported ELISA test kit is a critical step in ensuring repeatable results and should be taken care of by the operator. Whether it is manual or machine operation, incorrect results are often associated with incorrect washing, which relies on washing to achieve separation of free and bound enzyme labels. By washing, substances which are not able to bind to solid antigens or antibodies remaining in the pores of the plate, and interfering substances which are non-specifically adsorbed to the solid phase carrier during the reaction are eliminated.
3, incubation
Each reagent has its own *appropriate reaction mode in which temperature and incubation time control are important factors. Due to the high incubation temperature and long reaction time, the whole plate has a high background and a high positive rate. The incubation usually uses a wet box or a water bath, and the reaction plates should not be stacked to ensure that the temperature of each plate can be quickly balanced. To avoid evaporation, the plate should be covered.
4, microplate reader interpretation
As an instrument for recording the measurement results, the performance of the microplate reader is stable or not, and the reliability of the result is determined. First, the microplate reader should be regularly maintained, and the filter should be regularly calibrated; secondly, the microplate reader should be set to the correct wavelength, using dual wavelengths, one detection wavelength, and one reference wavelength to eliminate scratches and unevenness at the bottom of the microplate. Light interference caused by fingerprint or liquid level differences. In addition, when reading with the microplate reader, first wipe the bottom of the microplate and press the flat strip. Due to the different performance of various microplate readers, the instructions should be read in detail during use.
Due to the lack of standardization of the technology in enzyme-linked immunosorbent technology, some standard serum or reference serum (serum plate) is currently available in China. However, due to limitations in methodological and technical conditions, it is inevitable that certain non-specificity will occur in the enzyme-linked adsorption assay. However, we can reduce the specificity of detection by reducing the non-specific coloration to the bottom limit. And get more accurate and reliable experimental results.