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The use and use of the microplate reader
What is the use of the microplate reader? And how to use the microplate reader correctly? The following will be explained in detail by Shanghai Bayu technical staff:
First, the use of microplate reader:
The enzyme labeling instrument is also called enzyme-linked immunosorbent detector, which is a special instrument for enzyme-linked immunosorbent assay. The function of the microplate reader is to read the reaction result of the enzyme-linked immunosorbent kit. The test items include hepatitis B and AIDS detection. Eugenics and eugenics series testing, hormone testing, etc.
Second, the use of microplate reader:
first step
Connect the power supply, open the switch on the back of the machine, after the machine is turned on, after the self-test, according to the prompt on the machine, enter 00000 and press the enter key to enter the operation interface of the machine.
Second step
If the test program has been programmed, you can directly call up the program in the store search menu and read the board directly. If the program being tested needs to be re-edited, edit the new test program by pressing the program settings in the Edit menu.
third step
Edit the new program: Press the Edit menu, first enter the program settings, there are several sub-menus that need to be edited: threshold setting, report type setting, standard setting, test mode setting, and enzyme plate layout setting. Qualitative tests generally require the threshold to be set. Quantitation is generally not required; qualitative tests generally do not require the setting of standards, but
The quantity needs to be set for the standard.
the fourth step
Threshold setting: There are several sub-items in the threshold setting: not used, constant, quality control, formula, ratio, etc. Formula Threshold: Move the cursor to the formula threshold. There are five formulas in the machine to choose from. Select the corresponding formula according to the instructions on the kit, and then press the input key continuously to enter the formula to modify the K value parameter and gray. The zone value ( the modification of the K value parameter and the gray zone value, according to the data given in the kit description), press the enter key after the modification. QC: This setting only needs to input the gray zone value within the single threshold and press input. The above two items are the most common places to be tested and need to be modified. For example, if you want to use the quantitative test, you can choose not to use it. Skip this setting.
Step 5 Report Type Setting
Select this option with the following sub-items: Raw Data, Absorbance, Limits, Matrix Value, Threshold, Curve, Concentration, Difference. Qualitative tests generally select raw data reports, absorbance reports, and threshold reports; while quantitative tests generally select, raw data reports, absorbance reports, concentration reports, and curve reports.
Selection method: Move the cursor to the report to be selected, press the selection button to select the type of report to be selected, and then press the selection button to cancel the type of report just selected.
Curve reports can only be printed when the printer is built-in or used in conjunction with a computer.
Step 6 Standard Setting
This setting is for quantitative testing, and this setting is not available for qualitative testing.
Standard setting: ( 1 ) Setting of standard information, including the number of standards, concentration, unit ( 2 ) The standard curve setting includes the setting of the curve type and the setting of the coordinate axis.
Number of standard products: You can set the number of 0 - 12 standard products, fill in the concentration of the given concentration in the concentration option; there are dozens of units available in the unit option, select according to the unit of known concentration Consistent units.
Curve setting: There are a variety of curves available in the machine for the fitting of the standard; setting of the axis: In this option, the X- axis and Y- axis of the coordinate axis can be set linearly or non-linearly.
The seventh step of the test mode setting
Test mode setting: In the optical measurement mode, the machine defaults to a single wavelength. Move the cursor to a single wavelength, æ‘ right arrow, that is, select the key to select dual wavelength, and then click it to become a single wavelength. Move the cursor to the wavelength, and the right arrow is the selection button to select back and forth in the wavelength parameters set in the machine.
Vibration setting: The machine defaults to on, moving the cursor to the on position, the right arrow turns off; then it is back on. At the time: use the number keys to type in the required time, vibration intensity: use the right arrow to select the middle, strong, weak.
Readings: There are two sub-items in the reading settings, namely reading speed and reading mode. Move the cursor to the reading speed option, which has a quick reading and a step-by-step reading. Use the right button to select between the two options. Fast reading single wavelength 6 seconds, dual wavelength 15 seconds; step reading: single wavelength 15 seconds, dual wavelength 30 seconds; move the cursor to the reading mode, there are two kinds of common readings and evaluation readings, right click in two Select back and forth in the options. After the above parameters are selected, press the Enter key to save the modified contents. The fast reading in the reading speed is suitable for the test with a large amount of test; the normal reading in the reading mode, the reading result is read once, and the reading is read four times.
The average value makes the read data more accurate.
The eighth step is to set the ELISA plate layout.
æ‘Enter the key to enter this menu, select the manual plate, æ‘ enter the key. The default microplate layout of the machine is that the first vertical row is a blank hole, indicated by B , the second vertical row is the standard product indicated by S ; the latter is the added sample, indicated by X.
Typesetting method: You want to set a hole, for example, set the first vertical blank hole to the standard 9 hole, if the upper left corner of the screen is “ F â€. Just move the cursor to the hole and then slam the key labeled STD , then the screen shows that the hole is now a standard
In the No. 1 hole, the conversion key on the table below, the letter on the screen changes to “ N â€, and the input O9 becomes the standard No. 9 hole. All holes
Settings can be set in this way. In the case where the letter in the upper left corner of the screen is " F ", the properties of a hole can be set, such as blank ( BLK key); standard ( STD key ); quality control ( QC key ) , sample ( SMP key) the positive control (CP key), a negative control (CN bond) and the like; in the upper left corner of the screen is the letter can modify the numbered holes in the case of "N", the range of 0--96. The letters " F " and " N " can be switched back and forth by the toggle button. If multiple tests are used in a row layout, the template can be saved and used directly when used. The number of standard settings must match the number of standard products in the previous standard information. save. After saving the method, after setting the layout of the plate, press the input button.
The ninth step of the test name setting
The machine has a default name. If you want to modify it to make it easier for you to remember, you can use the cursor up button ( AZ) and down button (ZA) to select the letters you can easily remember. The switch button can change the case.æ‘ Enter the key to save the modified name. After the above test is basically set up, press the start button on the target to read the board, and then print automatically after reading, no need to print the button.
The following explains the settings of other items.
Permission setting
Change password: Enter a new password and press the enter key
Change user: Enter a new user and password, and enter the key.
Program lock or release: move the cursor to select the program, use the right arrow to lock or release
Light film setting
The machine has a total of eight slots, which can be equipped with eight light-receiving pieces. The machine is equipped with four light-receiving sheets for 405 , 450 , 490 , and 630. If the user has
Need to add a light film. Insert directly into the slot, gently turn the wheel, there is no feeling of resistance, then in this interface, after you insert
Enter the wavelength value of the position of the light guide and press the input key to return to the previous interface.
Time setting: In this interface, you can set the year, month, day, and specific time, hour, minute, second, and so on.
Through the above introduction, do you know about the use and usage of the microplate reader? If you have any questions, please feel free to contact us!
The use and application method of the microplate reader is provided by Shanghai Bashu. For more information on the microplate reader, please visit: