The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination. Covid-19 Nucleic Acid Extraction Reagent,Nucleic Acid Extraction Reagent Kits,Dna Purification Kit,Rna Purification Kit Jilin Sinoscience Technology Co. LTD , https://www.jlgkscience.com
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.
Eight items of herbicides used in vegetable fields
In vegetable production, cultivating and weeding often account for more than 40% of the total management of the entire field. If chemical weeding is used, not only can we effectively control weeds, improve the quality and yield of vegetables, but we can also save a lot of weeding labor and reduce labor intensity, but we must do the following “eight items of attentionâ€. One should try to use drugs before seedlings. For chemical weeding in vegetable fields, soil treatment is often used, but it must be applied before sowing or before sowing. The application time should be a good weeding effect at the initial stage of weeds. This can avoid or reduce the pesticide contamination of vegetables and residues. Second, we must make the soil flat and moist. In the treatment of soil with herbicides, the soil requires that the soil surface be smooth, finely crushed and moistened. This will not only ensure the uniform application of pesticides, but also create favorable conditions for the weeds to germinate, so that the weeds will germinate in unison, thus achieving a single use of drugs to kill most of the pesticides. The purpose of weeds is to increase the weeding effect. Third, timely medication. General chemical herbicides have a good effect in the weeding period. When weeds grow to 9 to 10 centimeters or are administered after delivery, the effect is poor or even ineffective. Therefore, control of weeds should be carried out during the germination stage of weeds. Fourth, spray evenly. Poorly wettable chemical herbicides are prone to precipitation. Therefore, when spraying, shake it frequently to ensure even spraying, otherwise the concentration of the lower layer of the liquid is too high, which can easily cause injury. Fifth, it is necessary to reduce medication in the facility cultivation area. Facilities such as protected areas are cultivated with high soil temperature, high humidity, and fast and high efficacy. Therefore, the dosage should be reduced by 20% to 30% in the exposed vegetable fields so as to avoid causing phytotoxicity to the vegetables. Six to alternate medication. Note that it is not possible to use a single herbicide for a long period of time in order to prevent weeds from developing resistance and improve weed control. Seventh, we must cover the soil in time. Some chemical herbicides such as trifluralin are volatile and should be coated with soil immediately after application to ensure efficacy. Some of the herbicides see light decomposition failure, can not be exposed to the sun. So, immediately cover the soil after using the medicine. Eighth, we must use a herbicide with a short potency. According to the variety of vegetables and the length of the growing season, it is generally better to use herbicides with a suitable duration to reduce the time of residual contamination and ensure safety.