Donkey frozen thawed: 1. Remove the frozen fine tube from the liquid nitrogen tank and quickly thaw it in a 38°C water bath for 30 seconds. 2. Dry the frozen fine tube and load it. 3. Detection of frozen sperm vitality, above the vitality of 0.4 can be used for artificial insemination. Insemination method: 1. Preserve the female donkey in an appropriate period, sterilize its genital area, rinse with warm water after boiling, and dry with sterile gauze. 2. The inseminator's arm is disinfected with disinfectant, rinsed with warm water after boiling, and wiped with sterile gauze. 3. When inseminating, the inseminator is standing on the left side of the female donkey and carrying the sperm in the right hand. The fingers are tapered and slowly inserted into the vagina of the female donkey. The insemination gun is inserted into the mouth of the cervix 5 to 7 cm away. The left hand slowly pushes the semen into the donkey uterus. 4. Slowly pull out the insemination gun from his left hand and slowly pull his right hand out of the vagina of the female donkey, and continue to gently rub the uterine vulva. Personal Care,Hair Conditioner,Body Wash Shower Gel,Moisturizing Shower Gel Wuxi Keni Daily Cosmetics Co.,Ltd , https://www.kenidailycosmetics.com