Flow cytometry has been widely used in basic life science research to clinical medical research, covering cell biology, immunology, hematology, oncology, pharmacology, microbiology, genetics and other fields in various disciplines. effect. The traditional sheath flow system instrument is large in size, and the complicated software system requires special operation and maintenance, which greatly limits the use of flow cytometry in common experiments. Merck Life Sciences adheres to the consistent concept of innovation and breaks through the mindset of streaming research and development, bringing a revolutionary new generation of MuseTM all-round cell quality control instrument. The microcapillary loading system that eliminates the sheath flow allows for accurate cell counting and analysis of cell viability, as well as basic flow functions, which can be placed directly in the cell house or even in a clean bench without the need for maintenance. Equipped with a comprehensive pre-programmed protocol and high-quality pre-packaged kits, no complicated operations and experimental parameter settings are required. Built-in Pad version of the touch screen computer, guided software design, half an hour to learn to stream, bid farewell to complex streaming experiments. Second, the cell cycle detection Cell cycle refers to the whole process of the cell from the completion of a split to the completion of the next split. A cell cycle consists of two phases: the interphase and the division. The interval is further divided into three phases, namely, the pre-DNA synthesis phase (G1 phase), the DNA synthesis phase (S phase), and the late DNA synthesis phase (G2 phase). Cell division phase: M phase. The amount of DNA in the cells changes periodically as the cell cycle progresses. Apoptosis detection Apoptosis is an important indicator for detecting cell viability and growth status. It is an important link in the process of cell death and a diagnostic standard commonly used by researchers in basic and clinical research. Among the traditional detection methods, the most classic test is the Annexin V & Dead Cell assay. Fourth, Muse multicolor DNA damage detection V. Muse human CD4 T cell detection Merck Life Science and Technology Service Hotline: GMP ATS Injection, Tetanus Antitoxin, Tetanus Toxoid ,Tetanus Antitoxin Injection, Antitetanus, Refined Tetanus Antitoxinsupplier in China Tetanus Antitoxin,Tetanus Toxoid,Tetanus Antitoxin Injection,Antitetanus&Refined Tetanus Antitoxin FOSHAN PHARMA CO., LTD. , https://www.pharmainjection.com
MuseTM versatile cell quality controller comprehensively evaluates cell status from cell viability, cell cycle, apoptosis, etc., greatly improving the accuracy of subsequent experiments, and has other applications for flow detection, such as signal pathway analysis and immunophenotype. Analysis, etc., multi-faceted applications meet the needs of daily experiments, flow detection is no longer time-consuming and laborious. 
application:
First, the cell absolute vitality count detection:
In the process of in vitro cell culture, there are always some cells in the population that die for various reasons. The percentage of living cells in the total cells is the cell viability we often say. The most basic indicator in "cell quality control" is cell viability. MuseTM versatile cell quality controller not only can count the cells in absolute, but also provide accurate and objective cell viability data, providing accurate and objective statistical basis for “cell quality controlâ€.
The MuseTM Cell Counting and Vigor Assay Kit uses a patented dual fluorescent labeling dye, which is a nuclear dye and a cell viability dye, respectively. Nuclear dyes are membrane-permeable dyes that distinguish between cells and cell debris. Cell viability dyes are non-permeable dyes that bind to nuclear DNA in dead cells that are damaged by cell membranes or in cells that are in apoptotic state. Through the combination of these two dyes, it is possible to accurately distinguish living cells, apoptotic cells, dead cells and cell debris, and quantitatively evaluate the cell viability state. 
DNA content:
Interphase:
G1 (pre-DNA synthesis, 2C),
S (DNA synthesis period, 2C-4C),
G2 (late DNA synthesis, 4C).
Splitting period:
M phase (DNA is 4C).
In normal cells, phosphatidylserine (PS) is only distributed on the inner side of the lipid bilayer of the cell membrane, and cells undergo early apoptosis, and PS is turned from the inside of the lipid membrane to the outside. As a phospholipid-binding protein, Annexin V has a high affinity for PS, and it binds to the cell membrane of early apoptotic cells through PS exposed to the outside of the cell. Therefore, Annexin V is a sensitive indicator for detecting early apoptosis in cells. 7-AAD is a nucleic acid dye that does not penetrate the intact cell membrane, but in the late stage of apoptosis and dead cells, 7-ADD can bind to the nucleus through the cell membrane. The cell membranes of normal cells and early apoptotic cells are intact and cannot bind to 7-AAD. The use of Annexin V in combination with 7-AAD distinguishes early-stage cells from late-stage cells and dead cells. Based on this principle, Muse's versatile cell quality controller provides an optimized premix kit, Muse Annexin V & Dead Cell Kit, which allows easy and rapid assessment of apoptosis. 
In addition to comprehensive control of cell survival quality through apparent characteristics and genetic alterations, Muse's versatile cell quality control device has designed a series of signal pathway kits to facilitate researchers to induce changes in the process of "cell quality control". Comprehensive research.
The DNA damage response is a very conservative mechanism of DNA damage induced by external and intrinsic factors. A network of multiple signal pathologies monitors and transmits the impairment signal and forms an appropriate response mechanism. Including the effects of cell cycle checkpoints and regulation of gene expression. Used in DNA damage response, tumor research, genetic research and other fields. The Muse Multicolor DNA Damage Detection Kit utilizes phosphorylated ATM (Ser1981) and H2A.X (Ser139) antibodies to quickly and easily detect activation of intracellular ATM and H2A.X and obtain percent activation results. 
T lymphocyte subsets can undergo abnormal changes in many clinical diseases, especially in patients with impaired immune function. Determination of T lymphocyte subsets changes have important implications for understanding the pathogenesis of the disease, guiding clinical treatment, and controlling the occurrence and development of the disease. For patients with cellular immunodeficiency, CD4+ is significantly reduced, often with a CD4+/CD8+ ratio inversion. This indicator is now commonly used for the monitoring of people living with HIV. The Muse Human CD4 T Cell Assay Kit detects the number and percentage of CD4 T lymphocytes. Simple and convenient operation for quick and accurate results. 
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