Animal anesthesia machine related to anesthesia knowledge When anesthesia machines use anesthesia, there are many kinds of drugs for general anesthesia through intravenous, intraperitoneal or intramuscular injection. The following are the most convenient, economical and safe, and the most widely used. Â Morris water maze analysis system experimental method: Training step content Acquired training ( 1 ) Put the head of the animal (rat or mouse) into the water toward the pool wall, and place it in one of four starting positions of east, west, south and north. Record the time ( s ) at which the animal found the underwater platform . In the previous training sessions, if this time exceeds 60s , the animal is guided to the platform. Let the animal stay on the platform for 10s. ( 2 ) Remove and dry the animals. Animals (especially rats) were baked under a 150 W incandescent lamp for 5 min , if necessary, and returned to the cage. Each animal was trained 4 times a day , with 15 to 20 minutes between training sessions and 5 days of continuous training . Exploration training On the second day after the last acquisition of sexual training, the platform was removed and the 60s exploration training began . The animals were placed in the water from the opposite side of the original platform quadrant. Record the time spent by the animal in the target quadrant (the quadrant where the platform was originally placed) and the number of times it entered the quadrant as a measure of spatial memory. Counterpoint training Determination of animal working memory (working memory). On the second day after the exploratory training, the four- day match training was started . Place the platform in the opposite quadrant of the quadrant where the original platform is located, in the same way as acquired training. Train 4 times a day . Each time you find the platform's time and swimming distance and swimming speed. Alignment training The second day of the last match training was conducted. The method is similar to the above exploration training. Record the time spent by the animal in the target quadrant (the second time the platform is located) and the number of times the animal entered the area within 60 seconds . Freeze-dried powder is a sterile powder injection obtained by freezing the liquid medicine into a solid state in a sterile environment, and subliming and drying the water in a vacuum. Freeze-dried powder is composed of a bottle of high-purity and high-active biological protein freeze-dried powder and a high-purity liquid essence. When using, it needs to be connected with a patented vacuum to reconstitute the freeze-dried powder and the essence to activate the biological protein activity. Freeze Dried Fruit Powder,Strawberry Powder,Freeze Dried Powder,Raspberry Powder YT(Xi'an) Biochem Co., Ltd. , https://www.ytnutra.com
(1) chloral hydrate: a sedative-hypnotic drug that can be used for intravenous anesthesia, but it has a large side effect of inhibiting respiratory and myocardial contraction. Combined with magnesium sulfate, sodium pentobarbital and alcohol, it can significantly reduce side effects and improve safety. Â
(2) Barbiturates: including sodium pentobarbital, sodium thiopental, sodium phenobarbital, and the like. These drugs can be administered intravenously or intraperitoneally, or in combination with other anesthetic drugs to alleviate the side effects of various drugs when used alone. Â
(3) Chemically pure alcohol: general anesthesia can be produced by intravenous injection, but the effect of anesthesia is weak, the dose required for letting the dog to sleep is large, and the time is long, and the time required to enter the systemic paralysis from the sleepy period is short and difficult to control. Therefore, it is not safe to use alone, and is generally used in combination with other anesthetic drugs. It is rarely used clinically. Â
(4) "846" mixture: also known as Suianxin, a mixture of several sedatives and anesthetics, containing Baodingning, haloperidol and other ingredients, is a wide range of anesthesia complex preparations. It has central analgesic, sedative and muscle relaxation effects. Satisfactory anesthesia can be achieved by intramuscular, intraperitoneal or intravenous injection alone. Â The principle of the Morris Water Maze experiment (Morris Water Maze)