Animal anesthesia machine related to anesthesia knowledge and morris water maze analysis system experimental method

Training step

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Acquired training

( 1 ) Put the head of the animal (rat or mouse) into the water toward the pool wall, and place it in one of four starting positions of east, west, south and north. Record the time ( s ) at which the animal found the underwater platform . In the previous training sessions, if this time exceeds 60s , the animal is guided to the platform. Let the animal stay on the platform for 10s.

( 2 ) Remove and dry the animals. Animals (especially rats) were baked under a 150 W incandescent lamp for 5 min , if necessary, and returned to the cage. Each animal was trained 4 times a day , with 15 to 20 minutes between training sessions and 5 days of continuous training .

Exploration training

On the second day after the last acquisition of sexual training, the platform was removed and the 60s exploration training began . The animals were placed in the water from the opposite side of the original platform quadrant. Record the time spent by the animal in the target quadrant (the quadrant where the platform was originally placed) and the number of times it entered the quadrant as a measure of spatial memory.

Counterpoint training

Determination of animal working memory (working memory). On the second day after the exploratory training, the four- day match training was started . Place the platform in the opposite quadrant of the quadrant where the original platform is located, in the same way as acquired training. Train 4 times a day . Each time you find the platform's time and swimming distance and swimming speed.

Alignment training

The second day of the last match training was conducted. The method is similar to the above exploration training. Record the time spent by the animal in the target quadrant (the second time the platform is located) and the number of times the animal entered the area within 60 seconds .