Spinal fixation system consists of posterior spinal fixation system,minimally invasive spinal system,posterior cervical fixation system,anterior cervical plate system,laminar shapping plate system and interbody fusion cage system.
Cervical and lumbar segmental implants are the most common types of spinal internal fixation, which can be roughly divided into anterior and posterior internal fixation according to their fixed positions and surgical approaches.
The anterior cervical vertebra is mostly fixed with locking plates and fixed screws,what are mostly made of titanium alloy materials; The posterior approach was fixed using the pedicle screw and rod system.
In some cases of spinal vertebrae bone defect, it is also necessary to implant titanium cage or PEEK cages to promote bone fusion of adjacent vertebrae. The titanium mesh cage refers to a cage shaped container made of titanium alloy material, which is loaded with autologous or allogeneic bone and placed in the spinal vertebrae bone defect, which not only serves as a strength support but also plays a role in bone fusion.
spinal fracture,Pedicle Screws,Spinal Implant,Spine Implants Jiangsu Aomed Ortho Medical Technology Co.,Ltd , https://www.medthofixation.com
1) After receiving the cells, please check the condition of the delivery culture bottle. If the culture bottle is damaged, there is liquid overflow and the cells are contaminated, please contact us after taking pictures.
2) When confirming the cell growth state under the microscope, it is performed under a low power microscope (4 or 5X objective lens) to accurately determine the cell passage density. Look at the morphology of the cells under the 10X and 20× objective lenses, and take 2-3 photos of the cells at the same time (10×, 20× take photos) as the basis for after-sales.
3) After observing the cell state, the T25 bottle is placed in a 37 °C incubator for about 2-3 hours.
4) Adherent cells: The adherent cells will fall off during transportation. If the adherent cells are found to fall off or fall off, the T25 bottle can be placed in a 37 ° C incubator for about 2-3 hours, and then extracted. The medium in the bottle and the non-adherent cells were centrifuged at 1000 rpm for 5 minutes, the supernatant was discarded, and inoculated into a primary culture flask (or a new culture flask) supplemented with a complete medium newly prepared according to the instructions for cell culture conditions.
5) Suspension cells: T25 bottles are placed in a 37 ° C incubator for about 2-3 hours, then the medium in the bottle and the cells are centrifuged at 1000 rpm for 5 minutes, the supernatant is discarded and resuspended, and inoculated into a new culture flask (add in accordance with the instructions. Newly prepared complete medium for cell culture conditions).
6) Remarks: The medium for transport (perfusion medium) can no longer be used to culture cells. Please use the complete medium newly prepared according to the cell culture conditions of the instructions to culture the cells. After receiving the cells, pass the passage of recommendation 1:2.
Cell use: For research use only.
The company's cell culture operating procedures for reference
One. Preparation of culture medium and culture cryopreservation conditions:
1) Prepare DMEM medium; high quality fetal bovine serum, 10%; double antibody, 1%.
2) Culture conditions: Gas phase: air, 95%; carbon dioxide, 5%. Temperature: 37 ° C, incubator humidity of 70% -80%.
3) Cryopreservation solution: 90% serum, 10% DMSO, ready to use.
two. Cell processing:
1) Resuscitation cells: Place the cryotube containing 1 mL of cell suspension into a 37 ° C water bath (the water surface is lower than the frozen tube lid), shake and thaw, and transfer to a prepared 15 ml centrifuge tube containing 4 mL of medium. well mixed. Centrifuge at 1000 RPM for 4 minutes, discard the supernatant, add 1 mL of medium, and mix well. All cell suspensions were then transferred to culture flasks containing 5 ml of medium for overnight incubation. The next day, change the fluid and check the cell density.
2) Cell passage: If the cell density reaches 80%-90%, subculture can be carried out.
For adherent cells, pass the following methods:
1. Discard the culture supernatant and rinse the cells 1-2 times with PBS containing no calcium or magnesium ions.
2. Add 2ml of digestive juice (0.25% Trypsin-0.53mM EDTA) to the culture flask, digest for 1-2 minutes in a 37 °C incubator, and observe the cell digestion under the microscope, if the cells are mostly rounded and shedding Quickly take it back to the console, tap the culture flask a few times, and then add 3 ml of this cell to stop the digestion.
3. Gently blow and aspirate, transfer to a 15 ml centrifuge tube, centrifuge at 1000 RPM for 4 minutes, discard the supernatant, add 1 mL of the culture solution, and mix well.
4. Transfer to a previously prepared T-25 flask containing 5 ml of medium or a T-75 flask containing 14 ml of medium.
3) Cell cryopreservation: When cells are in good growth state, cryopreservation of cells can be performed.
The following T25 bottle is an example;
1. When the cells were frozen, the medium was discarded, and the bottom of the bottle was washed 1-2 times with PBS, and then 1 ml of trypsin was added. After the cells were rounded off, the digestion was terminated by adding 2 ml of complete medium, which can be counted using a hemocytometer.
2.1000 RPM was centrifuged for 5 minutes to remove the supernatant. Resuspend with serum and add DMSO to a final concentration of 10%. After adding DMSO, mix well and distribute it to the cryotubes in the amount of 1 ml. Note that the cryotubes are labeled. The company freezes the number of cells per frozen tube more than 1X106 cells.
3. Place the cryotube in the program cooling box, put it into the -80 degree refrigerator, and transfer it to liquid nitrogen for storage at least 2 hours later. Record the location of the cryotube for the next time.
Precautions:
1. After receiving the cells, if you find that the dry ice has evaporated, the cap of the cryotube is detached, damaged, and the cells are contaminated, please contact us immediately.
2. All animal cells are considered to be potentially biohazardous and must be operated in a secondary biosafety station. Please pay attention to protection. All waste liquids and vessels that have been exposed to the cells need to be sterilized before disposal.
Culture procedure and precautions for mouse neural stem cells (C17.2)
Treatment of mouse neural stem cells (C17.2) cells after acceptance: