Application of FluorCam Chlorophyll Fluorescence Imaging System in Insect Crop Interaction Research

Recently, Beijing Yiketai Ecological Technology Co., Ltd. installed a FluorCam closed chlorophyll fluorescence imaging system for the Institute of Zoology of the Chinese Academy of Sciences. The system can be used to study the photosynthetic structure and photosynthetic activities of plants. The imaging function can visualize the metabolic state of all leaves and whole plants, and interpret the heterogeneity of leaf photochemical efficiency. The research group of the Institute of Zoology of the Chinese Academy of Sciences will use the FluorCam chlorophyll fluorescence imaging system and photosynthetic apparatus to study the interaction of aphid-tobacco and other insect crops.
The FluorCam closed chlorophyll fluorescence imaging system consists of a control unit and a dark-adapted box with an imaging area of ​​up to 13x13cm. The dark adaptation box has 4 LED light source boards, CCD lens, filter wheel and filter. The photochemical light of the installed system contains red light (620nm) and blue light (470nm), which can provide maximum light intensity of 2000μE and 3000μE respectively; saturated light is blue light (470nm), up to 6000μE.
The system is highly integrated, and the hardware operation is simple. The professional image software can also automatically run the program to capture the fluorescent dynamic video and automatically analyze the video image, generate the imaging map of common fluorescence parameters, and support the custom program writing and self-control. Define parameter imaging.
During the training, the indoor potted plants were used for demonstration. Two leaves of the same plant were selected: one leaf was large, the tip of the blade was withered and there was visible damage near the main vein; the other leaf was small, healthy, and the leaves were not withered. Or scars. After a short dark adaptation, run the Quenching fluorescence quenching program, and use the automatic region selection function of the image software to image the common fluorescence parameters of the two automatically generated regions (corresponding to two leaves). The parameters are as follows.
Data analysis showed that the maximum photochemical quantum efficiency (Fv/Fm), actual photochemical quantum efficiency (φPSII), and fluorescence decay rate (Rfd) of healthy leaves were higher than that of damaged leaves, and steady-state fluorescence (Fs) Low, non-photochemical quenching coefficient (NPQ) is equivalent.
In addition to providing an average of photosynthetic activity and photosynthetic parameters for the entire leaf, the chlorophyll fluorescence image also shows us the heterogeneity of photosynthetic characteristics in a single leaf. For damaged leaves, the chlorophyll content of the leaf tip is weak due to the decrease of chlorophyll content (indicated by false color "blue" in the figure), and the steady-state fluorescence Fs is higher in the damaged part (near the vein). (The figure is indicated by a false color "red"). The Fv/Fm, NPQ, and Rfd values ​​of the withered and damaged parts were lower than those of the normal part.
In recent years, chlorophyll fluorescence imaging technology has been widely used in the study of the effects of insect mites on photosynthetic characteristics of leaves due to its accurate measurement and visual expression of leaf photosynthetic heterogeneity.
The difference in photosynthetic characteristics between the leaves of the Phyllostachys pubescens and the normal part of the leaves caused by the genus Eriocheiridae (Fv / Fm of the photosynthetic activity of the locust part is low, and the actual photochemical quantum efficiency under light (φPSII) And the fluorescence decay rate ( Rfd ) is also low, which means that its ability to resist stress is poor)
The chlorophyll fluorescence imaging of the leaves of the genus Myrtaceae caused by the insects of the genus Mosquito (the parameters of Fv / Fm and φPSII of the photosynthetic activity of the larvae were lower, and the parameter Rfd of the activity of the leaves was also significantly reduced; the use of Rfd was effective. Analyzing and measuring the biological stresses caused by insects.)

As an expert in animal energy metabolism measurement, Yiketai Eco Technology Co., Ltd. provides you with complete animal energy metabolism and experimental medicine solutions:
  • Terrestrial animal energy metabolism and behavioral activity observation and research system
  • Aquatic animal respiratory metabolism and behavioral observation research system
  • Human Energy Metabolism Measurement System and Spectral Imaging Technology (Thermal Imaging and Hyperspectral Imaging)

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